HCS (High content screening) are key technology for number of phenotypic project who allow automatic measurement and analysis during time of different Biological event in 2D and 3D model. There is also rapidly growing need to use more physiologically relevant in vitro cell culture models. These include primary cells, patient-derived cells, human iPSC-derived disease-modeling cells, co-cultures of different types of cells and 3D cultures such as spheroids. To develop and advance phenotypic assays and screenings using these physiologically-relevant cellular models, new types of instruments needed to be developed to facilitate high-throughput fluorescence imaging and measurement of heterogeneous 2D and 3D cell cultures. A Light-Sheet Microplate Cytometer enables to obtain 3D fluorescence images of multiple 3D cellular objects with various sizes and morphologies in a well. A single focus-free scan is done for a whole plate. There are no need to focus on each cellular object and to take multiple xy-plane images of the object. In this context, we would like to introduce during the workshop this new technology to the community with 2D and 3D model for fast screening of size and morphology of these models. In order to facilitate questions, understand expectations, lead discussions, users will respond throughout the workshop to mini-quiz in real time via application on smartphones.