Clearing technics are used in biology to make initially opaque samples transparent and allow their three-dimensional imaging in depth. Many clearing protocols have been developed over the past twenty years, however these methods are continually modified according to the nature of the samples, their size, the fluorescent markings used and the imaging techniques used to analyze structures or follow the biodistribution of agents at the organ level. Most of clearing methods remove lipids stored in the tissue which is not compatible with 3D investigation of adipose tissue. In this workshop we will propose a method to clear adipose tissue and with the preservation of lipids. Dual fluorescent labeling of adipocytes and extracellular matrix will be developed before clearing and we demonstrate the high potentialities of combination of clearing and light-sheet fluorescence microscopy to image 3D structure of an entire adipose tissue.