This flash workshop is the 5 th of 6 different others, presenting 6 different applications of the commercial 3D software Imaris, from visualization, to network description. Immune cells adhesion and motitily play a pivotal role for immune response. Interestingly, change of morphology to reach a motility-associated shape is a consequence of T cell migration. The goal of this Flash tutorial, part of 6 others, is to follow immune cell trajectories, and cell shape changes over time. Participants will follow an analysis pipeline that used both the spots (cells), and the surfaces (cells or other extended structures) to study their distribution in term of cellular modification over time. The workshop will be a hands-on-session with a computer for each attendant, working with the same datasets (2D / time Spinning disk confocal images). They will follow a step by step a guiding workflow.