Light-sheet fluorescence microscopy (LSFM) is now a key method for high-speed volumetric imaging with applications in cell biology, neurosciences, developmental biology, organoids or plant research. Nevertheless, despite all these possibilities, problems such as alignment, sample adaptability and equipment control are often a barrier for potential users. Therefore, this complementary and easily adaptable imaging modality is not yet widespread over the bioimaging community. This workshop aims to introduce participants to the assembly of a compact and versatile light sheet microscope that can be combined with standard imaging systems. Attendants will assemble from scratch a 3D printed analogue module from which they can target the critical points of the design of a light sheet microscope. A user interface will be provided to characterize the light-sheet profile and perform volumetric acquisitions of various biological samples.