A044 - Structured illumination microscopy with a chip based light source

Andrea Bassi (andrea1.bassi@polimi.it)
Alessia Candeo (alessia.candeo@polimi.it)

The workshop will focus on the technique of Structured Illumination Microscopy (SIM), a widely used method for obtaining high and super-resolution images of biological specimens. The workshop will cover how to convert a standard widefield microscope into a SIM setup using a compact, integrated optical device that employs phase-shifted waveguides as a light source. Participants will gain hands-on experience with the SIM optical setup, alignment, and image acquisition process. Additionally, the workshop will provide training on the reconstruction of SIM images using a dedicated napari plugin, a python-based software for image visualization and analysis. The plugin is designed to be user-friendly, enabling participants with both programming and non-programming skills to utilize the software.