A007 - Label-free correlative microscopy of 3D samples: from light sheet to confocal microscopy

Laetitia Pieruccioni (laetitia.pieruccioni@cnrs.fr)
Corinne Barreau (corinne.barreau@inserm.fr)

Many imaging modalities are now available. These techniques are generally complementary and can be used to improve the speed of acquisition, the image resolution or the signal-to-noise ratio. Correlative imaging offers the advantage of being able to combine several microscopy techniques on the same sample and to extract the specificities of each, thus freeing oneself from these compromises. In the workshop we propose, we have developed a pipeline to large and complex sample, unlabeled samples with light sheet microscopy to get a 3D view of the entire sample, then image the same sample with confocal microscopy and re-match the two images.