The goal of the workshop is to explain the new technologie of the RIM ( Random Illumination Microscopy) and the interest for life science applications [1,2]. The principle of RIM is based of the knowledge of the statistical properties of the illumination. RIM is a super-resolution technique that extends the resolution of a factor in 3 dimensions of space, in a robust and quantitative way. RIM's inherent simplicity and extended biological applicability, particularly for imaging at increased depths, could help make SRM accessible to biology laboratories A new real-time algorithm will be presented, making RIM compatible with medium-content applications in life imaging. We illustrate the potential of RIM on diverse biological applications, from the mobility of chromatin in U2OS cells to the 3D motion of myosin minifilaments deep inside Drosophila tissues. The system is compatible with magnifications from X20 to X100. All imaging modalities will be available to participants during the workshop. [1] Mangeat, T., Labouesse, S., Allain, M., Negash, A., Martin, E., Guénolé, A., ... & Sentenac, A. (2021). Super-resolved live-cell imaging using Random Illumination Microscopy. Cell Reports Methods, 1(1), 100009. [2] Labouesse, S., Idier, J., Sentenac, A., Mangeat, T., & Allain, M. (2021, January). Random Illumination Microscopy from Variance Images. In 2020 28th European Signal Processing Conference (EUSIPCO) (pp. 785-789). IEEE.