This workshop aims to present an imaging approach for a high-resolution study of organs. The expansion microscopy method increases the resolution by 4 and circumvent the lack of funding to acquire a super resolution system. Clearing is the most common method for studying organs. This workshop proposes to show the contribution of expansion microscopy in comparison to clarification for studying organs. We are using clearing and expansion microscopy to image whole mouse lymph nodes by light sheet microscopy. Expansion microscopy allows us to reach a resolution to visualize structures in the whole organ in a way that we were unable to achieve with clearing. In this workshop, we show that microscopic expansion could be adaptable to whole organs, more specifically to the lymph node.